Journal: Aging (Albany NY)

Article Title: Brevilin A shows an anti-tumor role in prostate cancer via the lncRNA H19/miR-194/E2F3 signaling pathway

doi: 10.18632/aging.204744

Figure Lengend Snippet: The impact of Brevilin A on lncRNA H19, miR-194, and E2F3 expressions. DU145 cells were treated at the concentrations of 10 μM and 20 μM, which were calculated through the IC 50 values of Brevilin A. ( A – I ) qRT-PCR determined lncRNA H19 ( A – C ), miR-194 ( D – F ) and E2F3 mRNA ( G – I ) expressions. ( J – L ) Western blot measured E2F3 expression. * P < 0.05, ** P < 0.01, *** P < 0.001 (vs. control), n = 3.

Article Snippet: Primary anti-E2F3 antibody (1:100) (Proteintech, Wuhan, China) or Ki67 antibody (1:100) (Abcam, ab15580, MA, USA) was added for maintaining at 4°C overnight.

Techniques: Quantitative RT-PCR, Western Blot, Expressing, Control

Journal: Aging (Albany NY)

Article Title: Brevilin A shows an anti-tumor role in prostate cancer via the lncRNA H19/miR-194/E2F3 signaling pathway

doi: 10.18632/aging.204744

Figure Lengend Snippet: The profiles of lncRNA H19, miR-194, and E2F3 in prostate cancer tissues and cell lines. ( A , B ) qRT-PCR detected lncRNA H19 expression in prostate cancer tissues and cell lines. ( C , D ) qRT-PCR determined miR-194 expression in prostate cancer tissues and cell lines. ( E , F ) qRT-PCR examined E2F3 mRNA expression in prostate cancer tissues and cell lines. ( G , H ) Western blot was performed for assaying E2F3 protein level. ( I ) Immunohistochemistry checked E2F3 expression in prostate cancer tissues. Scale bar = 50 μm. * P < 0.05, ** P < 0.01, *** P < 0.001 (vs. Normal or PrEC), n = 3.

Article Snippet: Primary anti-E2F3 antibody (1:100) (Proteintech, Wuhan, China) or Ki67 antibody (1:100) (Abcam, ab15580, MA, USA) was added for maintaining at 4°C overnight.

Techniques: Quantitative RT-PCR, Expressing, Western Blot, Immunohistochemistry

Journal: Aging (Albany NY)

Article Title: Brevilin A shows an anti-tumor role in prostate cancer via the lncRNA H19/miR-194/E2F3 signaling pathway

doi: 10.18632/aging.204744

Figure Lengend Snippet: The interaction among lncRNA H19, miR-194, and E2F3. ( A , B ) Starbase database the binding site between lncRNA H19 and miR-194 as well as the binding site between miR-194 and E2F3. ( C – F ) qRT-PCR examined the profiles of lncRNA H19 and miR-194. ( G , H ) Dual luciferase reporter gene assay measured luciferase activity of DU145 cells. ( I , J ) The miR-194 level was probed via qRT-PCR. ( K – R ) Western blot and qRT-PCR tested the effects of lncRNA H19 and miR-194 on E2F3 expression. ns P > 0.05, *** P < 0.001, n = 3.

Article Snippet: Primary anti-E2F3 antibody (1:100) (Proteintech, Wuhan, China) or Ki67 antibody (1:100) (Abcam, ab15580, MA, USA) was added for maintaining at 4°C overnight.

Techniques: Binding Assay, Quantitative RT-PCR, Luciferase, Reporter Gene Assay, Activity Assay, Western Blot, Expressing

Journal: Aging (Albany NY)

Article Title: Brevilin A shows an anti-tumor role in prostate cancer via the lncRNA H19/miR-194/E2F3 signaling pathway

doi: 10.18632/aging.204744

Figure Lengend Snippet: The influence of lncRNA H19 and miR-194 on DU145 cell proliferation, apoptosis, invasion, and migration. H19 overexpression plasmids and si-H19 were transfected into DU145 cells, and miR-194 mimics or inhibitors were administered for 24 hours culture. ( A , B ) CCK8 assay detected cell proliferation. ( C , D ) Transwell monitored cell migration and invasion. Scale bar = 100 μm. ( E , F ) TUNEL staining examined apoptosis. Scale bar = 50 μm. ( G , H ) qRT-PCR examined the profiles of lncRNA H19 and miR-194. ( I , J ) Western blot was used for examining E2F3 protein level. ** P < 0.01, *** P < 0.001 (vs. NC or siNC); && P < 0.01, &&& P < 0.001 (vs. H19 or siH19), n = 3.

Article Snippet: Primary anti-E2F3 antibody (1:100) (Proteintech, Wuhan, China) or Ki67 antibody (1:100) (Abcam, ab15580, MA, USA) was added for maintaining at 4°C overnight.

Techniques: Migration, Over Expression, Transfection, CCK-8 Assay, TUNEL Assay, Staining, Quantitative RT-PCR, Western Blot

Journal: Aging (Albany NY)

Article Title: Brevilin A shows an anti-tumor role in prostate cancer via the lncRNA H19/miR-194/E2F3 signaling pathway

doi: 10.18632/aging.204744

Figure Lengend Snippet: The impact of miR-194 and E2F3 on DU145 cell proliferation, apoptosis, invasion, and migration. E2F3 overexpression and si-E2F3 plasmids were transfected into DU145 cells for 24 hour culture after miR-194 mimics or inhibitors were administered. ( A , B ) CCK8 assay checked cell proliferation. ( C , D ) Transwell assay examined cell migration and invasion. Scale bar = 100 μm. ( E , F ) TUNEL staining detected cell apoptosis. Scale bar = 50 μm. ( G , H ) qRT-PCR examined the profiles of lncRNA H19 and miR-194. ( I , J ) Western blot was used for examining E2F3 protein level. *** P < 0.001 (vs. NC or NC-in); & P < 0.05, && P < 0.01, &&& P < 0.001 (vs. miR-149 or miR-149 in), n = 3.

Article Snippet: Primary anti-E2F3 antibody (1:100) (Proteintech, Wuhan, China) or Ki67 antibody (1:100) (Abcam, ab15580, MA, USA) was added for maintaining at 4°C overnight.

Techniques: Migration, Over Expression, Transfection, CCK-8 Assay, Transwell Assay, TUNEL Assay, Staining, Quantitative RT-PCR, Western Blot

Journal: Aging (Albany NY)

Article Title: Brevilin A shows an anti-tumor role in prostate cancer via the lncRNA H19/miR-194/E2F3 signaling pathway

doi: 10.18632/aging.204744

Figure Lengend Snippet: Brevilin A hampered the pro-cancer function of lncRNA H19. Brevilin A was utilized to treat DU145 cells stably transfected with lncRNA H19 overexpression plasmids. ( A ) CCK8 assay checked cell proliferation. ( B ) Transwell assay examined cell migration and invasion. Scale bar = 100 μm. ( C ) TUNEL staining detected cell apoptosis. Scale bar = 50 μm. ( D , E ) qRT-PCR examined the profiles of lncRNA H19 and miR-194. ( F ) Western blot was used for examining E2F3 protein level. ** P < 0.01, *** P < 0.001 (vs. NC); & P < 0.05, && P < 0.01, &&& P < 0.001 (vs. H19), n = 3.

Article Snippet: Primary anti-E2F3 antibody (1:100) (Proteintech, Wuhan, China) or Ki67 antibody (1:100) (Abcam, ab15580, MA, USA) was added for maintaining at 4°C overnight.

Techniques: Stable Transfection, Transfection, Over Expression, CCK-8 Assay, Transwell Assay, Migration, TUNEL Assay, Staining, Quantitative RT-PCR, Western Blot

Journal: Aging (Albany NY)

Article Title: Brevilin A shows an anti-tumor role in prostate cancer via the lncRNA H19/miR-194/E2F3 signaling pathway

doi: 10.18632/aging.204744

Figure Lengend Snippet: The functions of Brevilin A and lncRNA H19 in the prostate cancer nude mouse xenograft model. DU145 cells, stably transfected with lncRNA H19 overexpression plasmids, were taken to construct a nude mouse xenograft model of prostate cancer, and Brevilin A was harnessed for treatment. The tumors were excised 28 days later. ( A ) The tumor volume. ( B ) The images of tumors. ( C ) The tumor masses. ( D , E ) Immunohistochemistry determined Ki67 and E2F3 expressions. ( F , G ) qRT-PCR examined the profiles of lncRNA H19 and miR-194. ( H ) Western blot was used for examining E2F3 protein level. *** P < 0.001 (vs. NC); && P < 0.01, &&& P < 0.001 (vs. H19), n = 5.

Article Snippet: Primary anti-E2F3 antibody (1:100) (Proteintech, Wuhan, China) or Ki67 antibody (1:100) (Abcam, ab15580, MA, USA) was added for maintaining at 4°C overnight.

Techniques: Stable Transfection, Transfection, Over Expression, Construct, Immunohistochemistry, Quantitative RT-PCR, Western Blot

Journal: Aging (Albany NY)

Article Title: Brevilin A shows an anti-tumor role in prostate cancer via the lncRNA H19/miR-194/E2F3 signaling pathway

doi: 10.18632/aging.204744

Figure Lengend Snippet: The functions of Brevilin A and lncRNA H19 in the prostate cancer nude mouse xenograft model. DU145 cells, stably transfected with lncRNA H19 overexpression plasmids, were taken to construct a nude mouse xenograft model of prostate cancer, and Brevilin A was harnessed for treatment. The tumors were excised 28 days later. ( A ) The tumor volume. ( B ) The images of tumors. ( C ) The tumor masses. ( D , E ) Immunohistochemistry determined Ki67 and E2F3 expressions. ( F , G ) qRT-PCR examined the profiles of lncRNA H19 and miR-194. ( H ) Western blot was used for examining E2F3 protein level. *** P < 0.001 (vs. NC); && P < 0.01, &&& P < 0.001 (vs. H19), n = 5.

Article Snippet: Primary anti-E2F3 antibody (1:100) (Proteintech, Wuhan, China) or Ki67 antibody (1:100) (Abcam, ab15580, MA, USA) was added for maintaining at 4°C overnight.

Techniques: Stable Transfection, Transfection, Over Expression, Construct, Immunohistochemistry, Quantitative RT-PCR, Western Blot